Experiment Three
In this experiment I compared the DNA extracted from different parts of a plant. I used the roots, the bulb, and the leaves of a leek. The temperature of alcohol was -15°C.
| DNA Source: Leek |
Observations (immediate) |
Observations (24 hours later) |
| Leek Roots | DNA has formed no apparent layer. The solution in test tube is really foamy. Approximately three minutes later a very thin layer formed where the alcohol and the leek roots meet. | DNA formed whitish cloudy particles at the bottom of the test tube. No DNA has formed anywhere else but at the bottom of the test tube. |
| Leek Bulb | DNA formed a long string of DNA from the alcohol to the top of the test tube | Formed cylinder shaped globs at the top of the test tube in a light greenish color. |
| Leek Leaves | DNA appeared immediately on the surface in light greenish blobs except for one test tube which was bumped. | In one test tubes DNA formed a long thick strand form top of the test tube to just above the leek leaves. In the other two test tubes small, thin root-like strands formed at the top of the test tubes. |
After the last written observations were made, DNA sources in each test tube were carefully removed and weighed.
Different parts of leek plant DNA in gm
Temperature of alcohol -15°C
|
Source |
1 |
2 |
3 |
Total in gm |
Average in gm |
|
Leaves |
1.43 |
1.46 |
1.46 |
4.35 |
1.45 |
|
Bulb |
1.26 |
1.14 |
1.23 |
3.63 |
1.21 |
|
Roots |
0 |
0 |
0 |
0 |
0.00 |