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DNA EXTRACTION

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Experiment Three

In this experiment I compared the DNA extracted from different parts of a plant. I used the roots, the bulb, and the leaves of a leek. The temperature of alcohol was -15C.

DNA Source: Leek

Observations

(immediate)

Observations

(24 hours later)

Leek Roots DNA has formed no apparent layer. The solution in test tube is really foamy. Approximately three minutes later a very thin layer formed where the alcohol and the leek roots meet.  DNA formed whitish cloudy particles at the bottom of the test tube. No DNA has formed  anywhere else but at the bottom of the test tube. 
Leek Bulb DNA formed a long string of DNA from the alcohol to the top of the test tube  Formed cylinder shaped globs at the top of the test tube in a light greenish color.
Leek Leaves DNA appeared immediately on the surface in light greenish blobs except for one test tube which was bumped. In one test tubes DNA formed a long thick strand form top of the test tube to just above the leek leaves. In the other two test tubes small, thin root-like strands formed at the top of the test tubes. 
 

After the last written observations were made, DNA sources in each test tube were carefully removed and weighed.

Different parts of leek plant DNA in gm

Temperature of alcohol -15C

 

Source

1

2

3

Total in gm

Average in gm

Leaves

1.43

1.46

1.46

4.35

1.45

Bulb

1.26

1.14

1.23

3.63

1.21

Roots

0

0

0

0

0.00

 

 

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